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Learn More About IFN Gamma ELISA

Interferongamma (IFNγ) is a cytokine which plays a significant role in causing and regulating a variety of immune reactions.  Cellular responses to IFNγ are mediated with its heterodimeric cellsurface receptor (IFNγR), which triggers downstream signal transduction cascades, finally resulting in the regulation of gene expression. 

To be able to study the function of IFNγ in several immune pathways and responses, researchers have developed mice with varied patterns of IFNγR receptor expression.These studies, in addition to investigations of naturally occurring mutations of those IFNγR in guy, are instrumental in elucidating the varied purposes of IFNγ. You can know more about IFN Gamma or IFNG or Interferon Gamma ELISA via Boster Bio.

The Individual Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of individual IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants.  IFN-γ is mainly secreted by T cells and NK cells, but can also be generated by dendritic cells and macrophages in smaller quantities. 

IFN-γ is a pleiotropic cytokine that modulates a wide range of immunological functions such as antimicrobial and antitumor action, and increases the surface expression of MHC class I and II proteins.  The assay relies in the sandwich ELISA system, where samples have been added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. 

The recorded cytokine is detected by means of a biotinylated detection antibody, followed closely by streptavidin-horseradish peroxidase, which contrasts the biotinylated antibody. Addition of this chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) leads to a coloured product using an intensity directly proportional to the concentration of cytokine from the sample.